The number of striatal cholinergic interneurons expressing calretinin is increased in parkinsonian monkeys.
Identifieur interne : 000167 ( Main/Exploration ); précédent : 000166; suivant : 000168The number of striatal cholinergic interneurons expressing calretinin is increased in parkinsonian monkeys.
Auteurs : Sarah Petryszyn [Canada] ; Thérèse Di Paolo [Canada] ; André Parent [Canada] ; Martin Parent [Canada]Source :
- Neurobiology of disease [ 1095-953X ] ; 2016.
Abstract
The most abundant interneurons in the primate striatum are those expressing the calcium-binding protein calretinin (CR). The present immunohistochemical study provides detailed assessments of their morphological traits, number, and topographical distribution in normal monkeys (Macaca fascicularis) and in monkeys rendered parkinsonian (PD) by MPTP intoxication. In primates, the CR+ striatal interneurons comprise small (8-12μm), medium (12-20μm) and large-sized (20-45μm) neurons, each with distinctive morphologies. The small CR+ neurons were 2-3 times more abundant than the medium-sized CR+ neurons, which were 20-40 times more numerous than the large CR+ neurons. In normal and PD monkeys, the density of small and medium-sized CR+ neurons was twice as high in the caudate nucleus than in the putamen, whereas the inverse occurred for the large CR+ neurons. Double immunostaining experiments revealed that only the large-sized CR+ neurons expressed choline acetyltransferase (ChAT). The number of large CR+ neurons was found to increase markedly (4-12 times) along the entire anteroposterior extent of both the caudate nucleus and putamen of PD monkeys compared to controls. Comparison of the number of large CR-/ChAT+ and CR+/ChAT+ neurons together with experiments involving the use of bromo-deoxyuridine (BrdU) as a marker of newly generated cells showed that it is the expression of CR by the large ChAT+ striatal interneurons, and not their absolute number, that is increased in the dopamine-depleted striatum. These findings reveal the modulatory role of dopamine in the phenotypic expression of the large cholinergic striatal neurons, which are known to play a crucial role in PD pathophysiology.
DOI: 10.1016/j.nbd.2016.07.002
PubMed: 27388937
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000129
- to stream PubMed, to step Curation: 000129
- to stream PubMed, to step Checkpoint: 000129
- to stream Ncbi, to step Merge: 002020
- to stream Ncbi, to step Curation: 002020
- to stream Ncbi, to step Checkpoint: 002020
- to stream Main, to step Merge: 000167
- to stream Main, to step Curation: 000167
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The number of striatal cholinergic interneurons expressing calretinin is increased in parkinsonian monkeys.</title><author><name sortKey="Petryszyn, Sarah" sort="Petryszyn, Sarah" uniqKey="Petryszyn S" first="Sarah" last="Petryszyn">Sarah Petryszyn</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author><author><name sortKey="Di Paolo, Therese" sort="Di Paolo, Therese" uniqKey="Di Paolo T" first="Thérèse" last="Di Paolo">Thérèse Di Paolo</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche du CHU de Québec, Faculty of Pharmacy, Université Laval, Quebec City, QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche du CHU de Québec, Faculty of Pharmacy, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author><author><name sortKey="Parent, Andre" sort="Parent, Andre" uniqKey="Parent A" first="André" last="Parent">André Parent</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author><author><name sortKey="Parent, Martin" sort="Parent, Martin" uniqKey="Parent M" first="Martin" last="Parent">Martin Parent</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC, Canada. Electronic address: Martin.Parent@fmed.ulaval.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27388937</idno><idno type="pmid">27388937</idno><idno type="doi">10.1016/j.nbd.2016.07.002</idno><idno type="wicri:Area/PubMed/Corpus">000129</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000129</idno><idno type="wicri:Area/PubMed/Curation">000129</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000129</idno><idno type="wicri:Area/PubMed/Checkpoint">000129</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000129</idno><idno type="wicri:Area/Ncbi/Merge">002020</idno><idno type="wicri:Area/Ncbi/Curation">002020</idno><idno type="wicri:Area/Ncbi/Checkpoint">002020</idno><idno type="wicri:Area/Main/Merge">000167</idno><idno type="wicri:Area/Main/Curation">000167</idno><idno type="wicri:Area/Main/Exploration">000167</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The number of striatal cholinergic interneurons expressing calretinin is increased in parkinsonian monkeys.</title><author><name sortKey="Petryszyn, Sarah" sort="Petryszyn, Sarah" uniqKey="Petryszyn S" first="Sarah" last="Petryszyn">Sarah Petryszyn</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author><author><name sortKey="Di Paolo, Therese" sort="Di Paolo, Therese" uniqKey="Di Paolo T" first="Thérèse" last="Di Paolo">Thérèse Di Paolo</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche du CHU de Québec, Faculty of Pharmacy, Université Laval, Quebec City, QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche du CHU de Québec, Faculty of Pharmacy, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author><author><name sortKey="Parent, Andre" sort="Parent, Andre" uniqKey="Parent A" first="André" last="Parent">André Parent</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author><author><name sortKey="Parent, Martin" sort="Parent, Martin" uniqKey="Parent M" first="Martin" last="Parent">Martin Parent</name><affiliation wicri:level="1"><nlm:affiliation>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC, Canada. Electronic address: Martin.Parent@fmed.ulaval.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Faculty of medicine, Université Laval, Quebec City, QC</wicri:regionArea><wicri:noRegion>QC</wicri:noRegion></affiliation></author></analytic><series><title level="j">Neurobiology of disease</title><idno type="eISSN">1095-953X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The most abundant interneurons in the primate striatum are those expressing the calcium-binding protein calretinin (CR). The present immunohistochemical study provides detailed assessments of their morphological traits, number, and topographical distribution in normal monkeys (Macaca fascicularis) and in monkeys rendered parkinsonian (PD) by MPTP intoxication. In primates, the CR+ striatal interneurons comprise small (8-12μm), medium (12-20μm) and large-sized (20-45μm) neurons, each with distinctive morphologies. The small CR+ neurons were 2-3 times more abundant than the medium-sized CR+ neurons, which were 20-40 times more numerous than the large CR+ neurons. In normal and PD monkeys, the density of small and medium-sized CR+ neurons was twice as high in the caudate nucleus than in the putamen, whereas the inverse occurred for the large CR+ neurons. Double immunostaining experiments revealed that only the large-sized CR+ neurons expressed choline acetyltransferase (ChAT). The number of large CR+ neurons was found to increase markedly (4-12 times) along the entire anteroposterior extent of both the caudate nucleus and putamen of PD monkeys compared to controls. Comparison of the number of large CR-/ChAT+ and CR+/ChAT+ neurons together with experiments involving the use of bromo-deoxyuridine (BrdU) as a marker of newly generated cells showed that it is the expression of CR by the large ChAT+ striatal interneurons, and not their absolute number, that is increased in the dopamine-depleted striatum. These findings reveal the modulatory role of dopamine in the phenotypic expression of the large cholinergic striatal neurons, which are known to play a crucial role in PD pathophysiology.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Petryszyn, Sarah" sort="Petryszyn, Sarah" uniqKey="Petryszyn S" first="Sarah" last="Petryszyn">Sarah Petryszyn</name></noRegion><name sortKey="Di Paolo, Therese" sort="Di Paolo, Therese" uniqKey="Di Paolo T" first="Thérèse" last="Di Paolo">Thérèse Di Paolo</name><name sortKey="Parent, Andre" sort="Parent, Andre" uniqKey="Parent A" first="André" last="Parent">André Parent</name><name sortKey="Parent, Martin" sort="Parent, Martin" uniqKey="Parent M" first="Martin" last="Parent">Martin Parent</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000167 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000167 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27388937
|texte= The number of striatal cholinergic interneurons expressing calretinin is increased in parkinsonian monkeys.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27388937" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ParkinsonCanadaV1
| This area was generated with Dilib version V0.6.29. |  |